Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/6447
Título: Identification of peptides from ovine milk cheese manufactured with animal rennet or extracts of Cynara Cardunculus as coagulant
Autor: Sousa, M. J.
Malcata, F. X.
Palavras-chave: Thistle flower
Cheese-making
Cheese ripening
Proteolysis
Enzyme activity
Data: 1998
Editora: American Chemical Society
Citação: SOUSA, M. J.; MALCATA, F. X. - Identification of peptides from ovine milk cheese manufactured with animal rennet or extracts of Cynara Cardunculus as coagulant. Journal of Agricultural and Food Chemistry. ISSN 0021-8561. Vol. 46 n.º 10 (1998), p 4034–4041
Resumo: Urea-PAGE of the water-insoluble extract (WISE) of ovine raw milk cheeses manufactured with proteinases of Cynara cardunculus or with commercial animal rennet indicated that the animal rennet acts more intensively, in quantitative terms, on ovine â-, Rs1-, and Rs2-caseins than the plant rennet. The water-soluble extract (WSE) from cheese produced by plant rennet was constituted by fragments of ovine â- and Rs2-caseins; peptides â-(f128-*), â-(f166-*), and â-(f191-*) were produced only by plant rennet, whereas peptides â-(f164-*) and â-(f191-*) were produced only by animal rennet. The peptide â-(f1-190) was identified as the primary product of ovine â-casein degradation in the WISE for both rennets. The complementary peptides Rs1-(f1-23) and Rs1-(f24-191) were produced by both rennets from ovine Rs1-casein; however, the bond Phe23-Val24 was cleaved by as early as 7 days in cheese manufactured with C. cardunculus, whereas 28 days had to elapse before that could be detected in the case of animal rennet. The peptide Rs1-(f24-165) was produced only by plant rennet, whereas the peptide Rs1-(f120-191) was produced only by animal rennet. The peptides produced from bovine Rs2-casein in cheese could not be traced as deriving from the action of proteinases from either rennet, so their existence is likely due to proteinases or peptidases released in cheese as a result of its indigenous microflora.
Peer review: yes
URI: http://hdl.handle.net/10400.14/6447
Versão do Editor: http://pubs.acs.org/doi/abs/10.1021/jf980188u
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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