Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/6441
Título: Hydrolysis of butteroil by immobilized lipase using a hollow-fiber reactor: part VI. Multiresponse analyses of temperature and pH effects
Autor: Malcata, F. Xavier
Hill, Charles G.
Amundson, Clyde H.
Palavras-chave: Immobilized lipase
Hollow fiber reactor
Lipolysis
Butteroil
Butterfat effects of pH
Effects of temperature
Multiresponse analysis
Data: 1993
Editora: Informa Healthcare
Citação: MALCATA, F. Xavier ; HILL, Charles G. ; AMUNDSON, Clyde H. - Hydrolysis of butteroil by immobilized lipase using a hollow-fiber reactor: part VI. Multiresponse analyses of temperature and pH effects. Biocatalysis and Biotransformation. ISSN 1024-2422. Vol. 8, n.º 3 (1993), p. 201-228
Resumo: A lipase from Aspergillus niger, immobilized by physical adsorption on hydrophobic hollow fibers made of microporous polypropylene, was used to effect the hydrolysis of the glycerides of melted butterfat at 40, 50, 55, and 60°C (pH 7.0), and at pH 3.0, 4.0, 5.0, 7.0, 8.0, and 9.0 (40°C). McIlvane buffer and melted butterfat were pumped cocurrently through the hollow fiber reactor. The concentrations of ten different free fatty acids in the effluent oil stream were measured by HPLC. Multiresponse nonlinear regression methods were employed to fit the data to multisubstrate rate expressions derived from a Ping Pong Bi Bi mechanism in which the rate controlling step is deacylation of the enzyme. Thermal deactivation of the immobilized lipase was also included in the mathematical model of reactor performance. A postulated normal distribution of vmax with respect to the number of carbon atoms of the fatty acid residue (with an additive correction for the number of double bonds) was found to provide the best statistical fit of the data. The models developed can be used to independently predict the effects of either the pH or the temperature, as well as the reactor space time and the time elapsed after immobilization, on the free fatty acid profile of the lipolyzed butteroil product.
Peer review: yes
URI: http://hdl.handle.net/10400.14/6441
Versão do Editor: http://informahealthcare.com/doi/abs/10.3109/10242429308998207
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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