Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/6422
Título: Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese production
Autor: Sampaio, Pedro N.
Calado, C.R.C.
Sousa, Lisete
Bressler, David C.
Pais, Maria Salomé
Fonseca, Luís Pina
Palavras-chave: Culture media composition
Cyprosin B
Fermentation
Response surface methodology
Saccharomyces cerevisiae
Data: 2010
Editora: Springer-Verlag
Citação: SAMPAIO, Pedro N. [et al.] - Optimization of the culture medium composition using response surface methodology for new recombinant cyprosin B production in bioreactor for cheese production. European food research and technology [Em linha]. 231:2 (2010) 339-346. Disponível na Internet: http://www.springerlink.com/content/ar5871t50476k014 . ISSN 1438-2385.
Resumo: The optimization of culture medium compositionwas carried out for improvement the recombinantcyprosin B production, an enzyme with high milk-clottingactivity. Response surface methodology (RSM) wasapplied to evaluate the effect of variables namely glucose,yeast extract (YE) and bactopeptone present in the culturemedium, used for recombinant cyprosin B production bytransformed Saccharomyces cerevisiae BJ1991 strain inshake-flask and bioreactor culture conditions. The centracomposite experimental design (CCD) was adopted toderive a statistical model for optimizing the composition ofthe fermentation medium. The optimal concentration estimatedfor each variable related to a theoretical maximumof cyprosin B activity (488 U mL-1) was 30 g L-1 glucose,15 g L-1 YE and 27 g L-1 bactopeptone. The optimizedmedium composition, based on empirical model, ledto a cyprosin B activity of 519 U mL-1, which correspondsto an increase of 46%. The fermentation using optimizedculture medium in a 5-L bioreactor allowed a significantincrease in biomass (82%) and recombinant cyprosin Bproduction (139%). The improvement in the recombinantcyprosin B production after optimization process can beconsidered adequate for large-scale applications, and theclotting activity of cyprosin B account for their use inindustrial cheese making.
Peer review: yes
URI: http://hdl.handle.net/10400.14/6422
ISSN: 1438-2385
Versão do Editor: http://www.springerlink.com/content/ar5871t50476k014
Aparece nas colecções:FE - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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