Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/6094
Título: Effect of heat on microorganisms isolated from freshly caught chilled scad (Trachurus trachurus)
Autor: Vaz-Pires, Paulo
Gibbs, Paul
Kirby, Roy M.
Palavras-chave: Damage sites
Fresh fish shelf-life
Growth parameters
Post-stress recovery
Data: 1996
Editora: Wiley Blackwell
Citação: VAZ-PIRES, Paulo ; GIBBS, Paul ; KIRBY, Roy M. - Effect of heat on microorganisms isolated from freshly caught chilled scad (Trachurus trachurus). International Journal of Food Science and Technology. ISSN 0950-5423. 31:3 (1996) 277-285
Resumo: The effects of heat on bacteria making up part of the microFlora of the sea-Fish Trachurus trachurus were studied. From 80 bacterial isolates which were subject to primary identiFication four were selected. The bacteria were subjected to a heat stress of 55°C for 20 s both in pure and mixed suspensions, the D-values and post-stress recovery in nutrient broth were measured. Using differential plating on selective agents to identify sites of injury and recovery times, the growth parameters dissolved oxygen, redox potential and pH were measured during recovery. Bacteria presumptively identiFied as Shewanella putrefaciens and Pseudomonas putida were more sensitive to heat than other bacteria, suggesting that most heat treatments of Fish may reduce the number of these bacteria. After dissolved oxygen was used up during recovery in nutrient broth, Eh declined from positive to negative values typical of facultative bacteria showing that, after initial aerobic growth, the bacteria changed their metabolism to grow anaerobically. When dissolved oxygen reached zero, about 120 h after inoculation, the numbers of all bacteria were about 107mL−1, and corresponds to a minimum in the pH values, except for the most heat sensitive isolate (300 h after inoculation). Differential plating showed increased sensitivity to NaCl induced by heat treatments in some isolates.
Peer review: yes
URI: http://hdl.handle.net/10400.14/6094
Versão do Editor: The definitive version is available at http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2621.1996.00336.x/abstract
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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