Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/5958
Título: Stability of a commercial lipase from Mucor javanicus: kinetic modeling of pH and temperature dependencies
Autor: Balcão, Victor M.
Oliveira, Teresa A.
Malcata, F. Xavier
Palavras-chave: Enzyme
PH
Lipolytic activity
Mathematical modelling
Batch reactor
Activation
Data: 1998
Editora: Informa Healthcare
Citação: BALCÃO, Victor M; OLIVEIRA, Teresa A.; MALCATA, F. Xavier -Stability of a commercial lipase from Mucor javanicus: kinetic modeling of pH and temperature dependencies. Biocatalysis and Biotransformation. ISSN 1024-2422. Vol. 16 (1998), p. 45-66
Resumo: The present communication reports experimental and modelling work pertaining to the independent roles of pH and temperature on deactivation of a crude lipase from Mucor javanicus. Experimental data oflipolytic activities were generated by a classic pH-stat assay on a triolein emulsion following incubation at several pH values for a fixed time, or at several temperatures for various times; postulated models were then fitted by nonlinear fitting to such data. The pH-dependence data were best fit by assumption of three forms of enzyme with increasing states of protonation, with pKa values of 6.2 and 11.3, respectively, where only the intermediate form is stable within the time frame considered. The thermal-dependence data were best fit by assumption of parallel steps of deactivation and rearrangement, with activation energies of 228.8 and 221.7 kJ mol~l, respectively.
Peer review: yes
URI: http://hdl.handle.net/10400.14/5958
Versão do Editor: http://informahealthcare.com/doi/abs/10.3109/10242429809040110?journalCode=bab
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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