Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/5866
Título: Spray drying as a method for preparing concentrated cultures of Lactobacillus bulgaricus
Autor: Teixeira, P.
Castro, H.
Kirby, R.
Data: 1995
Editora: Wiley
Citação: TEIXEIRA; P.; CASTRO, H.; KIRBY, R. - Spray drying as a method for preparing concentrated cultures of Lactobacillus bulgaricus. Journal of Applied Microbiology. ISSN 1365-2672. Vol, 78 n.º 4 (1995), p. 456-462
Resumo: Spray drying and freeze drying as methods for concentration of Lactobacillus bulgaricus starter cultures were compared in terms of viability,lag phase until onset of pH decrease and total acid production. For the experimental conditions used, no significant differences were detected between the methods. The effect of spray drying on the cell membrane of Lactobacillus bulgaricus was studied. Five separate methods were used to study the theory that spray drying causes cell membrane damage; three relating to leakage of intracellular components from the cell into the surrounding environment (260 and 280 nm absorbing materials, potassium ions and proteins) ; and two relating to increased cell permeability (increased sensitivity to NaCl and increased permeability to o-nitrophenyl-P-D-galactopyranoside (ONPG). Partial loss of some cytoplasmic material from the damaged cells was observed. The dried cells also became sensitive to NaCl and permeable to ONPG. Heat shock increased the survival of exponential cells as compared to controls but did not result in normal levels found with unshocked stationary phase cells. Heat shock had no effect on stationary phase cells. Different rehydration methods and media were investigated : slow rehydration increased survival.
Peer review: yes
URI: http://hdl.handle.net/10400.14/5866
Versão do Editor: The definitive version is available at: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.1995.tb03433.x/abstract
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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