Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/4900
Título: Distribution and characterization of Listeria monocytogenes clinical isolates in Portugal, 1994–2007
Autor: Almeida, G.
Morvan, A.
Magalhães, R.
Santos, I.
Hogg, T.
Leclercq, A.
Teixeira, P.
Data: 2010
Editora: Springer Verlag
Citação: ALMEIDA, G... [et al.] - Distribution and characterization of Listeria monocytogenes clinical isolates in Portugal, 1994–2007. European Journal of Clinical Microbiology and Infectious Diseases. ISSN 0934-9723. Vol. 29, n.º 10 (2010), p. 1219-1227
Resumo: In recent years, the number of cases of listeriosis has increased worldwide. Ninety-five isolates of Listeria monocytogenes recovered from Portuguese human cases of listeriosis have been characterized by biotyping (cadmium and arsenic sensitivity), polymerase chain reaction (PCR) grouping, and by pulsed-field gel electrophoresis (PFGE) applying the enzymes AscI and ApaI. Isolates were classified into one of three PCR groups; IVb (71.6%), IIb (17.9%), and IIa (10.5%). Four biotypes were differentiated: sensitive to arsenic/cadmium (48.4%), arsenic-sensitive and cadmium-resistant (25.3%), resistant to arsenic and sensitive to cadmium (18.9%), and resistant to both heavy metals (7.4%). Combined analyses of AscI and ApaI patterns yielded a total of 58 PFGE types with five sets (G, Jb, KKa, Me, and U) of Portuguese strains, each of which were indistinguishable by PFGE typing. In the present study, it was demonstrated that there are recurrent pulsotypes and that some were the same pulsotypes linked to outbreaks in France. In addition, there are some pulsotypes spread throughout the country, while others only appear in a restricted region. This study allowed the assembly of a first large pulsotype database of Portuguese clinical strains.
Peer review: yes
URI: http://hdl.handle.net/10400.14/4900
Versão do Editor: The original publication is available at http://www.springerlink.com/content/0224896h55xr7132/
Aparece nas colecções:CBQF - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
Distribution and characterization of listeria.1.PDF360,77 kBAdobe PDFVer/Abrir


FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.