Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/3315
Título: Study of cytolethal distending toxin (cdt ) in campylobacter coli using a multiplex polymerase chain reaction assay and its distribution among clinical and food strains
Autor: Fernandes, Mariana
Mena, Cristina
Silva, Joana
Teixeira, Paula
Data: 2010
Editora: Mary Ann Liebert
Citação: FERNANDES, Mariana …[et al.] - Study of cytolethal distending toxin (cdt ) in campylobacter coli using a multiplex polymerase chain reaction assay and its distribution among clinical and food strains. Foodborne Pathogens and Disease. ISSN 1535-3141. Vol. 7, n.º 1 (2010), p. 103-106
Resumo: From a collection of 242 food isolates of Campylobacter spp. 72 were identified as Campylobacter jejuni and 170 as Campylobacter coli and of 112 isolates from clinical sources, 92 were identified as C. jejuni and 20 as C. coli. The results for food isolates were in contrast to other reported identifications. Twenty C. coli strains from clinical cases of Campylobacter that occurred in Portugal and 170 isolated from poultry products were examined for the presence of cytolethal distending toxin (cdt) by multiplex polymerase chain reaction assay. CdtA, cdtB, and cdtC were present in 100% of the clinical strains. With respect to the food isolates, the cdtA and cdtC genes were detected in 99.4% of the strains and cdtB in 98.8%. Small point mutations or major deletions within the primer binding sites are possible explanations for these observations but need to be further investigated.
URI: http://hdl.handle.net/10400.14/3315
Versão do Editor: http://www.liebertonline.com/doi/abs/10.1089/fpd.2009.0326
Aparece nas colecções:CBQF - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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