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|Título:||Isolation of a xanthobacter sp. degrading dichloromethane|
|Autor:||Emanuelsson, Maria A. E.|
Osuna, M. Begoña
Jorge, Ruben M. Ferreira
Castro, Paula M. L.
|Citação:||EMANUELSSON, Maria A. E ...[et al.] - Isolation of a xanthobacter sp. degrading dichloromethane. Biodegradation. ISSN 1572-9729. Vol. 20, n.º 2 (2009), p. 235–244|
|Resumo:||A bacterial strain able to degrade dichloromethane (DCM) as the sole carbon source was isolated from a wastewater treatment plant receiving domestic and pharmaceutical effluent. 16S rDNA studies revealed the strain to be a Xanthobacter sp. (strain TM1). The new isolated strain when grown aerobically on DCM showed Luong type growth kinetics, with lmax of 0.094 h-1 and Sm of 1,435 mg l-1. Strain TM1 was able to degrade other aromatic and aliphatic halogenated compounds, such as halobenzoates, 2-chloroethanol and dichloroethane. The gene for DCM dehalogenase, which is the key enzyme in DCM degradation, was amplified through PCR reactions. Strain TM1 contains type A DCM dehalogenase (dcmAa), while no product could be obtained for type B dehalogense (dcmAb). The sequence was compared against 12 dcmAa from other DCM degrading strains and 98% or 99% similarity was observed with all other previously isolated DCM dehalogenase genes. This is the first time a Xanthobacter sp. is reported to degrade DCM.|
|Versão do Editor:||The original publication is available at http://www.springerlink.com/content/f13r3j721t172344/|
|Aparece nas colecções:||ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review|
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