Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/2857
Título: Effect of the pH of growth on the survival of Lactobacillus delbrueckii subsp. bulgaricus to stress conditions during spray-drying
Autor: Silva, J.
Carvalho, A. S.
Ferreira, R.
Vitorino, R.
Amado, F.
Domingues, P.
Teixeira, P.
Gibbs, P. A.
Palavras-chave: Heat shock proteins
Lactobacillus bulgaricus
pH growth conditions
Spray-drying
Storage
Thermotolerance
Data: 2005
Editora: Wiley Blackwell
Citação: SILVA, J...[et al.] - Effect of the pH of growth on the survival of Lactobacillus delbrueckii subsp. bulgaricus to stress conditions during spray-drying. Journal of Applied Microbiology. ISSN 1364-5072. Vol. 98, n.º 3 (2005), p. 775–782
Resumo: Aims: The aim of this study was to optimize survival of Lactobacillus delbrueckii subsp. bulgaricus during spraydrying and subsequent storage through optimizing the pH of growth conditions. Methods and Results: Cell concentrates previously grown without or with pH controlled were spray-dried and stored at 20ºC and heat treated at 57ºC. Cells grown under noncontrolled pH were more resistant to both drying and heating than cells grown under controlled pH but no significant differences were observed during storage. The intracellular proteins profile of cells grown under both conditions was studied by two-dimensional SDS-polyacrylamide gel electrophoresis. Eight proteins were identified using automated mass spectrometry (MS) and tandem mass spectrometry (MS/MS) data acquisition. Of the identified proteins, only cochaperonin GroES corresponded to a known heat shock protein (HSP). The other proteins identified are proteins involved in glycolysis. For cells grown under noncontrolled pH the expression of the Hsp70, GroES and GroEL, measured by Western blotting, was enhanced. Conclusions: The higher resistance of cells grown under noncontrolled pH correlates with the enhanced production of heat shock proteins. Significance and Impact of the Study: Growth of L. bulgaricus under controlled pH (commonly used by the starter cultures production industry) results in cells more sensitive to stresses frequently encountered by the cells during starter cultures preparation/storage/utilization.
URI: http://hdl.handle.net/10400.14/2857
Versão do Editor: The definitive version is available at http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2004.02516.x/abstract
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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