Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/2657
Título: Separation and identification of neutral cereal lipids by normal phase
Autor: Rocha, João M.
Kalo, Paavo J.
Ollilainen, Velimatti
Malcata, F. Xavier
Palavras-chave: Simple lipids
Acylglycerols
Free sterols
Fatty acids
Normal phase liquid chromatography
Evaporative light-scattering detection
Positive electrospray ionization tandem mass spectrometry
Data: 2010
Editora: Elsevier
Citação: "Journal of Chromatography A". ISSN 0021-9673. 1217: 28 (2010) 3013–3025
Resumo: A novel method was developed for the analysis of molecular species in neutral lipid classes, using separation by normal phase high-performance liquid chromatography, followed by detection by evaporative light-scattering and electrospray ionization tandem mass spectrometry. Monoacid standards, i.e. sterol esters, triacylglycerols, fatty acids, diacylglycerols, free sterols and monoacylglycerols, were separated to baseline on microbore 3 m-silica gel columns. Complete or partial separation of molecular species in each lipid class permitted identification by automatic tandem mass spectrometry of ammonium adducts, produced via positive electrospray ionization. After optimization of the method, separation and identification of molecular species of various lipid classes was comprehensively tested by analysis of neutral lipids from the free lipid extract of maize flour.
URI: http://hdl.handle.net/10400.14/2657
Versão do Editor: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TG8-4YG1KY1-4&_user=2460358&_coverDate=04%2F30%2F2010&_alid=1483883854&_rdoc=1&_fmt=high&_orig=search&_origin=search&_zone=rslt_list_item&_cdi=5248&_sort=r&_st=13&_docanchor=&view=c&_ct=1&_acct=C000057417&_version=1&_urlVersion=0&_userid=2460358&md5=aeb4d087bd27a14efa5c592c70924763&searchtype=a
Aparece nas colecções:ESB - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review

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