Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/17568
Título: Characterization and inhibitory activity of chitosan on hyphae growth and morphology of Botrytis cinerea plant pathogen
Autor: Silva Júnior, S.
Stamford, N. P.
Lima, M. A. B.
Arnaud, T. M. S.
Pintado, Manuela
Sarmento, B. F.
Palavras-chave: Biopolymer
Chitosan
Antifungal activity
Fungal morphology
Electron microscopy
Data: 2014
Citação: SILVA JÚNIOR, S… [et al.] - Characterization and inhibitory activity of chitosan on hyphae growth and morphology of Botrytis cinerea plant pathogen. International Journal of Applied Research in Natural Products. ISSN 1940-6223. Vol. 7, n.º 4 (2014), p. 31-38
Resumo: Summary. Low and high molecular weight chitosan were tested in different concentrations and growth times with the aim to evaluate the inhibitory activity against Botrytis cinerea, a very important plant pathogen. Tested chitosans were characterized by vibratory spectroscopy and elementary analyzes to determine the deacetylation degree. In addiction molar mass was estimated by viscosity measuring. Scanning electron microscopy was utilized for antimicrobial activity observation. Results showed that both chitosans markedly inhibited fungal growth, which was effected by incubation time and chitosan concentration. Scanning electron microscopy observations revealed that chitosan induced changes in surface morphology. The present study show that chitosan is capable of inhibit the growth and cause serious damage to the cell structure of the B. cinerea, as well as have the ability to form an impervious layer around the cell. Therefore, chitosan could be considered as a potential alternative for synthetic fungicides. Industrial relevance. Ultrastructural analysis showed that chitosan is capable of causing serious damage to the cell structure of the B. cinerea, as well as have the ability to form an impervious layer around the cell. Chitosan could inhibit the growth of B. cinerea in vitro and consequently may be considered as a potential alternative in replacement of synthetic fungicides.
Peer review: yes
URI: http://hdl.handle.net/10400.14/17568
Aparece nas colecções:CBQF - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review



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