Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/17465
Título: Avaliação do contributo da expressão genética na tolerância à desidratação em Rosa × hybrida cultivada em humidade relativa elevada
Autor: Carvalho, D. R. A.
Vasconcelos, Marta
Koning-Boucoiran, C. F. S.
Krens, F. A.
Heuvelink, E.
Carvalho, S. M. P.
Palavras-chave: Abscisic
acid
Metabolism
Stomata
Water stress
Data: 2014
Citação: CARVALHO, D. R. A… [et al.] - Avaliação do contributo da expressão genética na tolerância à desidratação em Rosa × hybrida cultivada em humidade relativa elevada. In Jornadas Ibéricas de Horticultura Ornamental, VI, Valencia, Spain, 1‐3 Octubre 2014. ROCA FERRERFÁBREGA, Dolors …[et al.] (ed.) - Las Buenas Prácticas en la Horticultura Ornamental: Actas de Horticultura, nº 68. 2014. ISBN 978-84-617-3020-9. Cap. 3, p. 132-137
Resumo: Cultivation of cut flowers with high postharvest longevity is of extreme importance for consumer’s satisfaction. A negative water balance is indicated as the most important factor reducing longevity. This problem is more severe in plants grown at high relative air humidity (i.e. RH > 85%) mainly due to a poor stomatal functioning associated to lower abscisic acid (ABA) concentrations, leading to higher water loss. In this paper it is analysed whether a sensitive cut rose cultivar to high RH show different gene expression related to ABA metabolism when grown at moderate (60%) or high (90%) RH. RNA was isolated from fully developed leaves, harvested 180 min after the beginning of the dark period (stomatal closing stimulus) and the relative gene expression was evaluated in genes involved in: biosynthesis (AAO3 and NCED1), oxidation (CYP707A1 and CYP707A3) and conjugation (UGT75B2) of ABA. The stomatal conductivity in the dark period was 5.6-fold higher in intact plants developed at high RH compared to moderate RH-grown plants, showing the reduced closure capacity of the stomata during darkness. After 4 h of leaflet desiccation, leaflets grown at high RH showed a relative water content (RWC) 69% lower than the moderate RH-grown leaflets, validating the sensitivity of K023 to high RH during growth. At last, from the five studied genes only the expression of CYP707A3 was significantly reduced by high RH, without significant differences found in the other genes. In conclusion, the sensitivity of the genotype K023 to high RH cannot be explained by the expression of the genes evaluated in this study, using leaves harvested 180 min after the beginning of the dark period (closing stimulus). We expect that after transferring the plants to the postharvest conditions, inducing water stress, a more contrasting gene expression among RH levels will appear.
Peer review: yes
URI: http://hdl.handle.net/10400.14/17465
Aparece nas colecções:CBQF - Artigos em actas / Papers in proceedings

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