Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.14/10415
Título: Effect of the metals iron, copper and silver on fluorobenzene biodegradation by Labrys portucalensis
Autor: Moreira, Irina S.
Amorim, Catarina L.
Carvalho, M. F.
Ferreira, António César da Silva
Afonso, Carlos M.
Castro, Paula M. L.
Palavras-chave: Iron
Copper
Silver
Fluorobenzene
Biodegradation
Labrys portucalensis
Data: 2013
Editora: Springer
Citação: MOREIRA, Irina S... [et al.] - Effect of the metals iron, copper and silver on fluorobenzene biodegradation by Labrys portucalensis. Biodegradation. ISSN 1572-9729. Vol. 24, n.º 2 (2013), p. 245-255
Resumo: Organic and metallic pollutants are ubiquitous in the environment. Many metals are reported to be toxic to microorganisms and to inhibit biodegradation. The effect of the metals iron, copper and silver on the metabolism of Labrys portucalensis F11 and on fluorobenzene (FB) biodegradation was examined. The results indicate that the addition of 1 mM of Fe2+ to the culture medium has a positive effect on bacterial growth and has no impact in the biodegradation of 1 and 2 mM of FB. The presence of 1 mM of Cu2+ was found to strongly inhibit the growth of F11 cultures and to reduce the biodegradation of 1 and 2 mM of FB to ca. 50 %, with 80 % of stoichiometrically expected fluoride released. In the experiments with resting cells, the FB degraded (from 2 mM supplied) was reduced ca. 20 % whereas the fluoride released was reduced to 45 % of that stoichiometrically expected. Ag+ was the most potent inhibitor of FB degradation. In experiments with growing cells, the addition of 1 mM of Ag+ to the culture medium containing 1 and 2 mM of FB resulted in no fluoride release, whereas FB degradation was only one third of that observed in control cultures. In the experiments with resting cells, the addition of Ag+ resulted in 25 % reduction in substrate degradation and fluoride release was only 20 % of that stoichiometrically expected. The accumulation of catechol and 4-fluorocatechol in cultures supplemented with Cu2+ or Ag+ suggest inhibition of the key enzyme of FB metabolism—catechol 1,2-dioxygenase.
Peer review: yes
URI: http://hdl.handle.net/10400.14/10415
Aparece nas colecções:CBQF - Artigos em revistas internacionais com Arbitragem / Papers in international journals with Peer-review



FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.